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Test Code ROTAV Rotavirus Antigen, Feces

Methodology

Enzyme Immunoassay (EIA)

Performing Laboratory

Huntsville Hospital Laboratory

Specimen Requirements

Specimen must arrive within 1 hour of collection.

 

Submit stool specimen in a clean, leakproof container. Collect specimen as follows:
1. Lift toilet seat, and cover toilet bowl with a large sheet of plastic wrap. Make a depression in plastic wrap. With toilet seat lowered, pass stool specimen directly onto plastic wrap.
2. Alternatively, collect stool specimen in a clean, dry, wide-mouth container. Do not use toilet paper to collect specimen. Do not remove specimen from toilet water.
3. If a stool specimen cannot be obtained on pediatric patients, insert a sterile swab approximately 1 inch (2.5 cm) beyond anal sphincter. Gently rotate swab to sample anal crypts. Collect 2 swabs (feces should be evident on swabs).

4. When using sterile swabs, follow instructions on swab packaging.
5. Label container/transport tube/swab tube (not packaging) with patient’s information, date and actual time of collection, and type of specimen.
6. Maintain sterility and forward promptly.

Note:  1. Maximum storage of specimen is 72 hours refrigerated.

2. Specimen will not be processed if transport/storage requirements are exceeded.
3. Contamination of stool specimen with urine or barium is not acceptable.

Reference Values

Negative (reported as positive or negative)
The virus is eliminated from the infected individual within a few days following acute infection. A prolonged carrier state has been recognized with rotavirus infection.

Day(s) Test Set Up

Monday through Sunday

CPT Coding

87425

Secondary ID

HHA1368

LOINC Coding

Test Name LOINC Code
Rotavirus Antigen, Feces 5880-0
Specimen 66746-9