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Test Code BPRP Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies

Reporting Name

Bordetella PCR

Useful For

Preferred diagnostic test for the detection of Bordetella pertussis or Bordetella parapertussis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Preferred:

Supplies:

Nasopharyngeal Swab (Rayon Mini-Tip Swab) (T515)

Specimen Type: Nasopharyngeal swab

Container/Tube: Rayon swab with an aluminum shaft placed in transport medium such as a green-top nasopharyngeal swab (rayon mini-tip) with Stuart's media (no charcoal) (T515), or Stuart's with charcoal, or Amies with or without charcoal (Transwab Nasopharyngeal with Charcoal System).

Additional Information:

1. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media. Clear semi-solid/solid media is gel and will be rejected.

2. Other swab or media types may be inhibitory to PCR testing and will be rejected.

 

Acceptable:

Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash

Container/Tube: Sterile container with a screw top cap (no transport media)

Specimen Volume: Entire collection


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Ambient  7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday, Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BPRP Bordetella PCR In Process

 

Result ID Test Result Name Result LOINC Value
SRC54 Specimen source 31208-2
34994 Bordetella pertussis PCR 43913-3
34995 Bordetella parapertussis PCR 42588-4

Reject Due To

Other Nose, nasal, or throat swab; calcium alginate or cotton-tipped swab; swab sent in gel transport medium, viral/universal transport medium, or Regan Lowe media; ESwab; swabs with solid plastic shaft; dry swab

Method Name

Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Includes DNA preparation from specimen, PCR amplification, and hybridization

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.